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KMID : 0613820140240030234
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Journal of Life Science
2014 Volume.24 No. 3 p.234 ~ p.241
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Isolation and Identification of a Novel Anticancer Compound from Solanum nigrum
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Yun Hee-Jung
Jung Jong-Hun
Hyun Sook-Kyung
Kim Byung-Woo
Kwon Hyun-Ju
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Abstract
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To identify and isolate anticancer active compounds from Solanum nigrum, S. nigrum was extracted with MeOH and then fractionated with various organic solvents (CH₂CI₂, EtOAc, n-BuOH, and H₂O). The cytotoxic effects of the MeOH extracts from S. nigrum and its organic solvent-soluble fractions were also tested in HT29 cells. All the MeOH extracts of S. nigrum and its organic-solvent extracts induced cytotoxicity in the HT29 cells. Among the extracts, H₂O was the most effective. The H₂O extract was purified further by repeated silica gel, Sephadex LH-20, Diaion HP- 20, and RP-18 column chromatography. An active anticancer compound, Des-N-26-methylene-dihydrotomatidine, was isolated with a molecular weight of 416 and a molecular formula of C_28H_48O₂. Analysis of the cytotoxic effects of Des-N-26-methylene-dihydrotomatidine on the HT29 cells compared to those of tomatine and tomatidine are similar in its structure, is higher than tomatidine above the 40 μg/ml concentration, but lower than tomatine. This is the first study to describe the anticancer activity of Des-N-26-methylene-dihydrotomatidin, isolated from S. nigrum. Des-N-26- methylene-dihydrotomatidine seems to have potential as a natural bioactive compound.
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KEYWORD
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Anticancer activity, anti-cancer active compound, Des-N-26-methylene-dihydrotomatidine, HT29 cells, Solanum nigrum
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